Analytical comparison of 11 CE-IVD marked real-time PCR assays for Borrelia burgdorferi sensu lato detection.

ABSTRACT:

Purpose

In some precise clinical manifestations of Lyme borreliosis, PCR can be helpful for diagnosis: suspicion of atypical erythema migrans, borrelial lymphocytoma, acrodermatitis chronica atrophicans, Lyme arthritis or in very early seronegative neuroborreliosis. This study aimed to evaluate: (1) the analytical sensitivity of commercial real-time PCR kits for Borrelia burgdorferi sensu lato (Bbsl), (2) their specificity, and (3) their practicability.

Methods

The performances of 11 CE-IVD marked commercial kits and the French NRC Borrelia's in-house PCR were evaluated. PCRs were performed blindly on quantified DNA extracts, from 14 Bbsl strains corresponding to eight different genospecies, and on DNAs from relapsing fever (RF) Borrelia, Treponema, Leptospira, and 26 other

SUBMITTER: Zilliox L 

PROVIDER: S-EPMC12619792 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature