Bioactive compound identification without fractionation: an Ocimum spp. case study.

ABSTRACT:

Introduction

Identifying the phytochemistry underpinning a plant's observed therapeutic benefits is essential for understanding mechanisms of action and developing novel therapeutics. More recent efforts fusing global metabolomics and multivariate predictive modeling have improved compound discovery; however, these models rely on chemical variations between samples, which often necessitates at least one round of fractionation and may result in compound loss or degradation.

Objectives

This study uses multiple whole botanical extracts to explore whether a metabolome-wide association study approach can accurately identify bioactive phytochemicals without prior fractionation.

Methods

We employed 40 Ocimum extracts with a range of IC₅₀ levels against HT-29 cells

SUBMITTER: Abraham EJ 

PROVIDER: S-EPMC12619805 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature