Identification of affinity-optimized peptide binders of a viral protease for chemical genetic applications.

ABSTRACT: Chemically-controlled genetic tools are useful for studying biological systems due to their ability to dose-dependently and temporally modulate intracellular function. Chemically-disrupted proximity (CDP) systems, which involve the pre-localization of two interacting protein components that can be disrupted with a small molecule, are complementary to more commonly used chemically-inducible dimerization (CID) systems. However, fewer CDP systems have been developed, and the genetically-encoded protein components have not been as optimized for intracellular applications. Here, we describe a transcriptional activation reporter assay for screening the intracellular interaction between the 21-amino acid peptide ANR and HCVp NS3a, which are the genetically-encoded components of a CDP system that

SUBMITTER: Banales-Mejia F 

PROVIDER: S-EPMC12619934 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature