Fiber Distribution and Myelination of Dopaminergic Neurons in the Medial Forebrain Bundle of a Rodent Depression Model.

ABSTRACT: It has been hypothesized that the rapid/long-lasting antidepressant action observed following medial forebrain bundle DBS in clinical trials of Treatment-Resistant Depression patients could partially be driven by modulation of the midbrain-to-forebrain dopaminergic fibers. The study investigated the spatial distribution and myelination status of dopaminergic fibers within the rodent equivalent structure in a preclinical depression model, the Flinders Sensitive Line (FSL). Fixed, sliced brain sections were double stained with anti-tyrosine hydroxylase/anti-dopamine-β-hydroxylase antibodies to distinguish dopaminergic from noradrenergic fibers, and with anti-tyrosine hydroxylase/anti-myelin antibodies to specifically investigate myelination. Quantification was done at six predefined segments

SUBMITTER: Duan Z 

PROVIDER: S-EPMC12619984 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature