Native interface of the SAM domain polymer of TEL.

ABSTRACT:

Background

TEL is a transcriptional repressor containing a SAM domain that forms a helical polymer. In a number of hematologic malignancies, chromosomal translocations lead to aberrant fusions of TEL-SAM to a variety of other proteins, including many tyrosine kinases. TEL-SAM polymerization results in constitutive activation of the tyrosine kinase domains to which it becomes fused, leading to cell transformation. Thus, inhibitors of TEL-SAM self-association could abrogate transformation in these cells. In previous work, we determined the structure of a mutant TEL-SAM polymer bearing a Val to Glu substitution in center of the subunit interface. It remained unclear how much the mutation affected the architecture of the polymer, however.

Results

Here we determine the structure

SUBMITTER: Tran HH 

PROVIDER: S-EPMC126206 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature