Developing a Modular Platform for the Detection of microRNAs Using Rolling Circle Amplification and Multi-Primed Chain Amplification.

ABSTRACT: MicroRNAs (miRNAs) play critical regulatory roles in diverse biological processes and are key biomarkers in a wide range of physiological and pathological conditions, including cancer. However, their inherently low concentrations in biological samples pose a major challenge for reliable detection and quantification. To overcome this limitation, we developed a fluorescence-based biosensing platform that integrates rolling circle amplification (RCA) and multi-primed chain amplification (MCA) to enhance signal and detection sensitivity. The system is engineered to allow flexible reconfiguration for different miRNA targets by altering probe and primer sequences. In this modular system, miR-i, a miRNA commonly expressed in healthy and cancerous samples, serves as a universal initiator for RCA.

SUBMITTER: Nasiri M 

PROVIDER: S-EPMC12620632 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature