The transcription factor BnaWRKY75 enhances resistance to the necrotrophic pathogen Sclerotinia sclerotiorum by promoting salicylic acid biosynthesis in oilseed rape.

ABSTRACT: Sclerotinia sclerotiorum, a devastating necrotrophic fungus causing stem rot in oilseed rape, results in significant global yield losses. Deciphering plant-S. sclerotiorum interactions is crucial for disease control. Here, we report that the transcription factor BnaWRKY75 positively regulates resistance to this pathogen. BnaWRKY75 was the most highly induced WRKY gene upon S. sclerotiorum infection. Overexpressing BnaA10.WRKY75 in both Arabidopsis and oilseed rape enhanced resistance to S. sclerotiorum, whereas BnaWRKY75 knockout plants exhibited reduced resistance. RNA sequencing and DNA affinity purification sequencing showed that the isochorismate synthase gene BnaICS1, a key gene required for salicylic acid (SA) biosynthesis, is a potential target of BnaA10.WRKY75. Yeast one-hybrid, du

SUBMITTER: Liu D 

PROVIDER: S-EPMC12621092 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature