CEPR1 function across plant lineages: peptide hormone recognition by cereal crop orthologs and developmental roles in barley.

ABSTRACT: Arabidopsis peptide hormones from the C-TERMINALLY ENCODED PEPTIDE (CEP) family and their receptor, CEP RECEPTOR1 (CEPR1), integrate growth and development with environmental cues. Since cereals display anatomical differences to dicots, it is unknown if CEPR1 functions similarly in monocots and dicots. We investigated cereal CEPR1 function by introducing putative barley, rice, or maize CEPR1 orthologs into an Arabidopsis cepr1 mutant to determine if its diverse root, vegetative development, and fecundity phenotypes could be restored. The monocot CEPR1 genes restored Arabidopsis root, shoot, seed phenotypes, and CEP DOWNSTREAM 1 expression. To validate cereal CEPR1 function, we knocked out CEPR1 in barley using CRISPR-Cas9. Barley cepr1 plants had steeper seminal roots, a narrower mature ro

SUBMITTER: Taylor K 

PROVIDER: S-EPMC12621094 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature