Rebalancing the seed proteome following deletion of vicilin-related genes in pea (Pisum sativum L.).

ABSTRACT: Null mutations for genes encoding a major seed storage protein in pea, vicilin, were sought through screening a fast-neutron mutant population. Deletion mutations at four or five vicilin loci, where all vicilin genes within each locus were deleted, were combined to address the question of how removal or reduction of a major storage protein and potential allergen might impact the final concentration of protein per unit of mature seed weight, seed yield, and viability. While the concentration of seed protein was not reduced in mature seeds of mutant lines, indicative of a re-balancing of the proteome, notable differences were apparent in the metabolite, proteomic, and amino acid profiles of the seeds, as well as in some functional properties. Major effects of the deletions on the proteome we

SUBMITTER: Rayner T 

PROVIDER: S-EPMC12621101 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature