FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single cells with fluorescence lifetime imaging microscopy.

ABSTRACT: Fluorescence lifetime imaging microscopy (FLIM) is a cellular-resolution molecular imaging technique. Yet, the journey from raw photon decays to biological insight remains fragmented by multi-step data extraction and siloed analyses. This work presents FLIM Playground, the first interactive graphical platform that unifies single-cell FLIM workflows, embeds user checks at each stage, and offers diverse user options. Built in Python and available open-source, FLIM Playground runs on major operating systems as a ready-to-run application and is web deployable. Its Data Extraction section collects and checks field-of-view metadata, calibrates via instrument response function shift or fluorescence lifetime standard, and extracts single-cell fluorescence lifetime features, along with morphology a

SUBMITTER: Zhao W 

PROVIDER: S-EPMC12621712 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature