A single-cell transcriptomic atlas of inner ear morphogenesis in zebrafish.

ABSTRACT: The inner ear constitutes different cell types next to one another: the sensory patches whose hair cells synapse with neurons, the thin channels of three semicircular canals whose perpendicular organization enables detection of directional head rotation, and the endolymphatic duct and sac whose conditional epithelial barrier relieves excess pressure and promotes fluid pressure homeostasis. How the ear's component cell states are established during development has remained unknown. We use single-cell RNA sequencing to distinguish cell states within the developing ear with wild-type zebrafish embryos and lmx1bb mutants that exhibit defects in canal and sac morphogenesis. We identify the earliest marker for the semicircular canal-genesis zone (ccn1l1), unexpected genes in the en

SUBMITTER: Swinburne IA 

PROVIDER: S-EPMC12621729 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature