Comparative transcriptomics reveals shifts in cortical architecture at the metatherian/eutherian transition.

ABSTRACT: The neocortex, a layered structure unique to mammals, supports higher-order functions such as perception, learning, and decision-making. While its overall organization is broadly conserved, it remains unclear whether changes to the cell type-specific organization of the cortical column-the basic unit of cortical processing- occurred at the metatherian/eutherian split. To address this, we used single-nucleus RNA sequencing and spatial transcriptomics to compare gene expression, cell types, and laminar architecture in the primary visual cortex of a metatherian (Monodelphis domestica) and a eutherian mammal (Mus musculus). We find distinctions between supragranular (layer 2/3) and infragranular (layers 5 and 6) intratelencephalic (IT) neurons to be more pronounced in mice, with

SUBMITTER: Gorzek R 

PROVIDER: S-EPMC12621739 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature