Mapping the Subtype-Specific PARP1 ADP-ribosylated Proteome in Breast Cancer Cells.

ABSTRACT: Breast cancers are molecularly heterogeneous, with subtype-specific differences in transcriptional programs, chromatin architecture, and therapeutic responses. While PARP1 has been extensively studied in the context of DNA repair, emerging evidence implicates its catalytic activity in a broader set of cellular processes, including the regulation of gene expression. Here, we employed an NAD analog-sensitive PARP1 (asPARP1) chemical genetics approach combined with mass spectrometry to map the ADP-ribosylated proteome across six human breast cancer cell lines representing luminal and basal/triple negative subtypes. We identified thousands of PARP1 substrates and hundreds of Glu/Asp ADPRylation sites, revealing both shared and subtype-specific modifications in cell lines maintained under basal

SUBMITTER: Koul S 

PROVIDER: S-EPMC12621782 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature