Finishing a complete giraffe genome from telomere to telomere with Verkko-Fillet.

ABSTRACT: High-quality genome assemblies are essential for understanding speciation, evolution, and for building reference genomes and pan-genomes. Despite major advances in long-read sequencing technologies and graph-based assembly algorithms, current assemblies often remain incomplete and require refinement, including correction of haplotype switching and resolution of gaps, particularly within repetitive regions such as ribosomal DNA clusters and recent segmental duplications. These limitations largely stem from challenges in graph curation and are not adequately addressed by conventional polishing methods, which focus primarily on nucleotide-level corrections. To overcome these issues, we developed Verkko-Fillet, a Python-based interactive framework for genome graph inspection, editing, and cura

SUBMITTER: Kim J 

PROVIDER: S-EPMC12621783 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature