Mechanisms of Differential Signal Transduction by IFNLR1 Variants.

ABSTRACT:

Background

Lambda interferons bind the interferon lambda receptor-1 (IFNLR1) and IL10RB heterodimer to induce interferon stimulated genes (ISGs) and impart antiviral immunity. We previously showed that protein variants derived from distinct IFNLR1 splice isoforms uniquely influence gene expression and HBV replication in stem cell-derived hepatocytes (iHeps). Here, we evaluated the molecular mechanisms of signal transduction by full-length canonical IFNLR1 (variant 1) and a noncanonical variant missing a portion of the Box1 and Box2 JAK1-interacting motifs (variant 2).

Methods

We used HEK293T cells, wild-type (WT), and IFNLR1 knock-out (KO) iHeps that stably express doxycycline-inducible, FLAG-tagged IFNLR1 variants to evaluate function. Cellular responses to IF

SUBMITTER: Novotny LA 

PROVIDER: S-EPMC12621819 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature