Independent Tuning of Stiffness and Pore Size in 3D Rat Tail Collagen I Matrices.

ABSTRACT: The interplay between the extracellular matrix (ECM) mechanical properties and the tumor microenvironment is increasingly recognized as a critical factor in cancer progression. Three-dimensional (3D) culture systems have emerged as essential platforms for in-vitro cell-based applications, offering microenvironments that are more physiologically relevant compared to traditional two-dimensional (2D) cultures. However, independently controlling the topological and mechanical features of 3D matrices remains challenging due to the interdependence of these parameters. In this study, we demonstrate a method for independently tuning pore size and stiffness in collagen I (Coll I) networks and examine their effects on breast cancer and epithelial cell morphology and cluster formation. Collagen conce

SUBMITTER: Vessella T 

PROVIDER: S-EPMC12621833 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature