Photoproximity labeling of c-Myc reveals SLK as a cancer specific co-regulator.

ABSTRACT: Transcription factors (TFs) have long been aspirational therapeutic targets for the treatment of diseases, as their dysregulation is a common mechanism for altered cell states. Despite this, many TFs implicated in disease have disordered structures and lack canonical binding pockets, rendering them non-trivial targets for small molecule-based therapies. Directly inhibiting TF function has proven difficult, but indirect inhibition by targeting the effector molecules that modulate TF function is a promising, yet underexplored, alternative approach. Here we report a strategy for capturing cancer-specific protein-protein interactions using context-dependent μMap photoproximity labeling. Using an intein-based method for catalyst conjugation in biochemically intact nuclei, we demonstrate that we

SUBMITTER: Milione RR 

PROVIDER: S-EPMC12621887 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature