Diversity and abundance of bacteria in an underground oil-storage cavity.

ABSTRACT: BACKGROUND:Microorganisms inhabiting subterranean oil fields have recently attracted much attention. Since intact groundwater can easily be obtained from the bottom of underground oil-storage cavities without contamination by surface water, studies on such oil-storage cavities are expected to provide valuable information to understand microbial ecology of subterranean oil fields. RESULTS:DNA was extracted from the groundwater obtained from an oil-storage cavity situated at Kuji in Iwate, Japan, and 16S rRNA gene (16S rDNA) fragments were amplified by PCR using combinations of universal and Bacteria-specific primers. The sequence analysis of 154 clones produced 31 different bacterial sequence types (a unique clone or group of clones with sequence similarity of > 98). Major sequence types we

SUBMITTER: Watanabe K 

PROVIDER: S-EPMC126219 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature