Intravital calcium imaging of meningeal macrophages reveals niche-specific dynamics and aberrant responses to brain hyperexcitability.

ABSTRACT: The meninges, which envelop and protect the brain, host a dense network of resident macrophages with diverse roles in regulating homeostasis and neuroinflammation. Despite their importance, we have a limited understanding of their behavior in vivo. Many dynamic cellular functions of macrophages involve intracellular Ca²⁺ signaling. However, to our knowledge, virtually nothing is known about the spatiotemporal Ca²⁺ dynamics of meningeal macrophages. We developed a chronic intravital two-photon imaging approach and related computational analysis tools to interrogate meningeal macrophage Ca²⁺ dynamics, at a subcellular resolution, in a novel Pf4^Cre:TIGRE2.0^GCaMP6s/wt reporter mouse model. Using imaging in awake mice, we characterized the

SUBMITTER: Carneiro-Nascimento S 

PROVIDER: S-EPMC12622066 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature