Pharmacokinetic and biodistribution analysis of monoclonal antibodies: a comprehensive study of antibody biodistribution and partitioning coefficients in mice.

ABSTRACT: The efficacy of therapeutic monoclonal antibodies (mAbs) often hinges on biodistribution to their site of action. However, traditional pharmacokinetic (PK) assessments - typically based on measuring plasma or total tissue concentrations - fail to reflect the interstitial concentrations that are most relevant for tissue targets. This study aimed to address this limitation by integrating experimentally determined vascular and interstitial volumes from tissues in SCID-beige mice with a comprehensive PK time-course and biodistribution analysis of four distinct anti-viral monoclonal antibodies (mAbs 1-4) with no endogenous mouse target. The biodistribution studies included 11 tissues, characterizing tissue and plasma concentrations over a 168-h time-course. Total and interstitial tissue concent

SUBMITTER: Rabow Z 

PROVIDER: S-EPMC12622353 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature