The fluorescent probe DISBAC2(3) provides a high-throughput screening tool for evaluating abiotic stress tolerance in plants.

ABSTRACT: The electric gradient across cellular membranes (termed membrane potential [MP]) provides a driving force for the uptake and translocation of all essential nutrients in their ionic form, as well as operating in stress sensing. MP is causally associated with abiotic stress tolerance in plants and, thus, could be used as a proxy in phenotyping programs. However, the conventional method to detect MP changes, which involves impaling the membrane with a microelectrode, requires specialized equipment and specialist skills, is highly time consuming, and is prone to various possible artifacts. Here, we present a high-throughput screening approach that enables the rapid detection of MP changes in plants using the fluorescent probe DISBAC2(3). Using 3 case studies (salinity stress and hypoxia stress

SUBMITTER: Ivsic C 

PROVIDER: S-EPMC12622375 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature