Vortex-Generated Microdroplets Enable Simple, Rapid, and Low-Volume Measurement of Condensate Volume and Concentration.

ABSTRACT: Biomolecular condensates are increasingly recognized for their crucial roles in intracellular organization and the origin of life. Despite their growing importance, efficiently and accurately quantifying condensate volume and concentration remains challenging, hindering the understanding of their properties and functions. Conventional spectroscopy-based methods for measuring condensate concentration in bulk systems have practical limitations; for example, they require laborious calibration and preparation procedures, large sample volumes, and in vitro analysis. Here, we introduce a microdroplet-based method that leverages a simple and rapid vortex-assisted emulsification approach to encapsulate condensates within microdroplets. By determining the microdroplet-to-condensate size ratio and p

SUBMITTER: Chen F 

PROVIDER: S-EPMC12622474 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature