Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass.

ABSTRACT: Lignocellulosic biomass is a sustainable feedstock for biorefineries, but inefficient xylose utilization limits microbial bioproduction. Here, the oleaginous yeast Yarrowia lipolytica was engineered to produce succinic acid (SA) from xylose by resolving metabolic and regulatory conflicts. Initial overexpression of xylose catabolic genes (XR, XDH, XK) in an SA-hyperproducing strain did not activate xylose utilization, indicating underlying cryptic constraints. Adaptive evolution identified critical mutations (Snf1^R78W, Scp1^delGTC) that globally downregulated downstream pathways, including glycolysis and β-oxidation, restoring growth using xylose but reducing SA production. To overcome this trade-off, a random expression library strategy incorporating multi-copy amplifi

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC12622525 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature