Marker Metabolite-Based Multi-Omics Analysis Identifies New Loci Controlling Thousand Seed Weight in Brassica Napus.

ABSTRACT: The thousand seed weight (TSW) of Brassica napus (B. napus) is a crucial agronomic trait, and metabolites serve as a bridge between genotype and phenotype. Leveraging previously released metabolome data from a 388 B. napus population, 137 marker metabolites significantly correlated with TSW are identified, and 21 markers significantly correlate with both TSW and seed oil content. To delve deeper into the relationships between selected marker metabolites and phenotypes, a comprehensive multi-omics analysis is conducted, which unveils 734 metabolite-QTLs, 5,225 expression-QTLs, and 9,077 transcriptome-wide significantly associated genes. Employing weighted correlation network analysis, a triple-link network is constructed involving 133 metabolites, 731 QTLs, and 3,321 genes. The multi-omics

SUBMITTER: Li L 

PROVIDER: S-EPMC12622543 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature