Intraoperative Bioprinting for Craniomaxillofacial Bone Reconstruction in Rats and Sheep.

ABSTRACT: Craniomaxillofacial reconstruction is challenging due to the requirement for diverse manual surgical interventions, which significantly increase as the defect volume enlarges. To address these concerns, we utilized intraoperative bioprinting (IOB) to reconstruct cranial bone defects in surgical settings. We formulated an innovative collagen-based bioink supplemented with human adipose-derived stem cells (hADSCs) or bone morphogenetic protein-2 (BMP-2). The concentration and dispersion state of collagen along with hADSCs were precisely adjusted to enhance cytocompatibility, bioprintability, and osteogenic activities. IOB was first performed via a 3-axis bioprinter on a rat model having a critical-sized calvarial defect (39.3 mm³), which was infilled within ≈30 s and resulted in ≈

SUBMITTER: Yeo M 

PROVIDER: S-EPMC12622550 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature