Differential effect of supercoiling on bacterial transcription in topological domains.

ABSTRACT: DNA supercoiling (SC), the over- and under-winding of DNA, is generated by transcription as described in the twin-domain model. Conversely, SC also impacts transcription through torsional stress. SC therefore regulates transcription dynamically and independently of transcription factor binding, particularly in the context of chromosomal topological domains and the activity of topoisomerases in bacteria. In this work, we develop numerical simulations of SC-coupled transcription of a single gene within a topological domain, based on a model incorporating stochastic transcription and activities of topoisomerase I and gyrase. We explore the effect of several parameters not systematically assessed in previous works (role of topoisomerase activities, topological domain size, gene expression stre

SUBMITTER: Goldberg B 

PROVIDER: S-EPMC12622846 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature