Dermal fibroblast cultures recapitulate differences between deermice and mice in their responses to a Toll-like receptor agonist.

ABSTRACT:

Introduction

The white-footed deermouse Peromyscus leucopus is a primary reservoir for the agents of Lyme disease and other zoonoses in North America and manifests infection tolerance for the bacteria, protozoa, and viruses it hosts. In previous in vivo studies, P. leucopus and Mus musculus differed in the degree of sickness and profiles of biomarkers after exposure to a bacterial lipopolysaccharide, a TLR4 agonist.

Methods

As an approach for assessing immunity of mammals in nature and for longitudinal studies of colony animals in the laboratory, we evaluated primary dermal fibroblast cultures of P. leucopus and M. musculus in their short-term responses to a TLR2 agonist lipopeptide using bulk and single-cell RNA-seq.

Results

By sing

SUBMITTER: Duong JV 

PROVIDER: S-EPMC12623179 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature