Meso-macroporous hydrogel for direct litre-scale isolation of extracellular vesicles.

ABSTRACT: Extracellular vesicles are cell-originated lipid bilayer membrane vesicles that play vital roles in cell-to-cell communications. While extracellular vesicles hold substantial biomedical potential, conventional methodologies for isolating extracellular vesicles require elaborate preprocessing and, therefore, remain labour intensive and limited by throughput. To overcome these challenges, we present a facile fabrication route for generating a meso-macroporous hydrogel matrix with pores of ~400 nm for customizable extracellular vesicle isolation. By combining surface charge-selective capture of extracellular vesicles within the hydrogel matrix and their recovery by high ionic strength, we report direct extracellular vesicle isolation with a throughput range from microlitre to litre scales, wi

SUBMITTER: Kim J 

PROVIDER: S-EPMC12623240 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature