Stereoscopic Quantitative Analysis of Enteric Nervous System in Patients With Slow Transit Constipation.

ABSTRACT:

Background

In previous studies, abnormal changes in the enteric nervous system (ENS) were often found in intestinal specimens from patients with slow transit constipation (STC). However, there are no clear pathological diagnostic criteria for STC due to the lack of accurate quantitative data references. The association of ENS alterations with STC remains unanswered.

Methods

Full-thickness colon specimens were obtained from 10 STC patients who underwent subtotal colectomy and 20 colon cancer patients who underwent radical colectomy. Using stereoscopic imaging combined with tissue clearing, immunohistochemistry, and confocal imaging techniques, the differences in ENS quantitative data between STC patients and controls were observed, and the correlation between this change and

SUBMITTER: Tian G 

PROVIDER: S-EPMC12623296 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature