Novel method for the identification of circulating SARS-CoV-2 variants and clinical characteristics of patient infection with SARS-CoV-2 variants in Central China.

ABSTRACT:

Introduction

We established a reliable and cost-effective method for identifying severe acute respiratory syndrome coronavirus 2 variants circulating in central China and analysed the clinical characteristics of patients with acute coronavirus disease 2019 who were infected with these variants.

Methods

The RNA of centrifuged and enriched samples was extracted and reverse transcribed into cDNA. cDNA was then analysed using a nested polymerase chain reaction amplification and Sanger sequencing method targeting specific mutations in the spike, ORF1a, and N genes. This was validated against next-generation sequencing, achieving 100% concordance.

Results

Among 172 isolates, JN.1.18.2 was the most prevalent (52.9%, 91/172), followed by XDV.1 (25.0%, 43/172), J

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC12623389 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature