Identification of Schistosoma mansoni gender-associated gene transcripts by cDNA microarray profiling.

ABSTRACT:

Background

Parasitic helminths of the genus Schistosoma mate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the infection are associated with this process. We have used cDNA microarray technology to initiate genome-wide gene-expression studies of sex and sexual development in mature Schistosoma mansoni parasites.

Results

An S. mansoni-specific cDNA microarray was fabricated using 576 expressed sequence tags selected from three cDNA libraries and originating from two different parasite developmental stages. Five independent cDNA microarray hybridizations were analyzed using stringent filtering criteria and careful quality control, leading to the identification of 12 new female-associa

SUBMITTER: Hoffmann KF 

PROVIDER: S-EPMC126235 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature