Refining the adjuvant-induced rat model of monoarthritis by optimizing the induction volume and injection site.

ABSTRACT: Arthritis is a highly prevalent and disabling condition characterized by pathological joint-damage, clinical symptoms of pain and loss of normal joint function. Monoarthritis can be modelled in rodents via intraarticular injection of Complete Freund's Adjuvant (CFA), inducing both joint inflammation and pain-like behaviours. This study aimed to compare the outcome of different injection-volumes and joint-locations, to refine the model's use and to improve its validity. Male and female Sprague Dawley rats were injected with CFA into the ankle (10, 20 or 50 µl) or knee joint (10, 50 or 100 µl), and assessed on dynamic weight bearing, locomotor activity, depressive- and anxiety-like behaviours, histology, and various welfare and model-specific parameters. Induction of monoarthritis resulted i

SUBMITTER: Berke MS 

PROVIDER: S-EPMC12623504 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature