Ex Vivo Confocal Laser Scanning Microscopy for Real-Time Pattern and Single-Cell Analysis in Inflammatory Skin Diseases.

ABSTRACT: Inflammatory skin diseases are common and often difficult to differentiate. Ex vivo confocal laser scanning microscopy (EVCM) offers a rapid and promising approach. This study aimed to assess the diagnostic utility of EVCM in differentiating inflammatory dermatoses, particularly eczema, psoriasis and lichen planus, by comparing its performance with gold standard histopathology. Tissue samples of 110 patients presenting with inflammatory skin conditions were subjected to both EVCM and conventional histopathology. EVCM images were analysed by three blinded observers, with varying knowledge in histopathology and EVCM, utilising pattern analysis based on Ackermann's classification and single-cell analysis focusing on neutrophil (neutrophils) and eosinophil (eosinophils) granulocytes. Sensitivi

SUBMITTER: Hartmann D 

PROVIDER: S-EPMC12623711 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature