M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis.

ABSTRACT: N6-methyladenosine (m⁶A) methylation modification can influence lung adenocarcinoma (LUAD) progression by regulating gene expression. Ferroptosis, a novel regulated cell death, is involved in various malignant progression. This study aimed to elucidate the role of ferroptosis-related long non-coding RNA (lncRNA) with m⁶A modification in LUAD. By integrating MeRIP-seq and RNA-seq data from in-house samples and public databases, we identified that lncRNA RBMS3-AS3 was abnormally downregulated in LUAD. The low expression of RBMS3-AS3 predicted poor prognosis in LUAD patients. RBMS3-AS3 was downregulated by m⁶A "eraser" ALKBH5 in an m⁶A-dependent manner. Reduced RBMS3-AS3 expression promoted LUAD progression in vitro and in vivo through inhibiting fe

SUBMITTER: Ge W 

PROVIDER: S-EPMC12623742 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature