Kinetic analysis of catalytic activity of G-quadruplex/hemin DNAzyme with flanking adenine nucleotides.

ABSTRACT: G-quadruplex/hemin DNAzymes are promising nucleic acid catalysts due to their versatility and ease of use in biosensing applications. Their peroxidase-like catalytic activity can be enhanced through various strategies, including modifications to flanking nucleotides. In this study, the catalytic effects of flanking nucleotide modifications at the 3' and 5' ends of the DNAzyme were investigated. Additionally, the structural topology of the G-quadruplex/hemin DNAzymes was characterized using circular dichroism (CD) spectroscopy. Similar to the unmodified DNAzyme, the modified G-quadruplex with adenine (A) nucleotide at the 3'-terminal extension adopted a parallel topology in the presence of hemin. After optimizing the reaction conditions, the kinetic parameters of both original and 3' flanki

SUBMITTER: Udomprasert A 

PROVIDER: S-EPMC12623798 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature