Alpinetin pretreatment prevents lipopolysaccharide/D-galactosamine-induced acute liver injury in mice by inhibiting ferroptosis via the Nrf2/SLC7A11/GPX4 pathway.

ABSTRACT: This study aims to investigate whether alpinetin prevents lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced acute liver injury (ALI) in mice by inhibiting ferroptosis, and to explore the potential molecular mechanisms involved. Thirty-six male BALB/c mice were randomly assigned to one of six groups (n = 6): the control group, the LPS/D-GalN group, the Ferrostatin-1 (Fer-1) + LPS/D-GalN group, and the alpinetin (12.5, 25, or 50 mg/kg) + LPS/D-GalN groups.The ferroptosis-specific inhibitor Fer-1 was used as a positive control to verify the involvement of ferroptosis in the injury. Following intraperitoneal (i.p.) administration of alpinetin (12.5, 25, or 50 mg/kg) for three consecutive days, acute liver injury was induced by an i.p. injection of LPS (30 µg/kg) and D-GalN (600 mg/kg). T

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12623886 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature