Loop structure in poly(A) tail of mRNA vaccine enhances antigen translation efficiency and mRNA stability.

ABSTRACT: As mRNA technology emerges as a groundbreaking innovation, an increasing number of studies are attempting to increase expression levels by optimizing the structure of mRNA, such as the 5' or 3' untranslated regions (UTR). We designed a novel loop structure in the poly(A) tail of an mRNA platform to improve the stability and duration. Protein expression analyses were conducted using bioluminescence, both in vitro and in vivo. Additionally, cellular and humoral responses to different antigens were confirmed using flow cytometry and ELISA of splenocytes and serum isolated from mice immunized with mRNA containing different poly(A) tail structures. The poly(A) tail with a loop structure exhibited higher bioluminescence signals, both in vitro and in vivo, and increased human erythropoietin (hEPO

SUBMITTER: Oh A 

PROVIDER: S-EPMC12623927 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature