Caloric restriction enhances inheritance of wild-type mitochondrial DNA in Saccharomyces cerevisiae.

ABSTRACT: In Saccharomyces cerevisiae, an asymmetrical division model, mitochondrial (mt) DNA typically exists in a homoplasmic state, but mutations frequently occur. Rolling-circle replication, mediated by the mtDNA recombinase Mhr1p, forms tandem concatemers that are selectively transmitted to budding cells. In crosses between haploids with wild-type (ρ⁺) and hypersuppressive (HS) ρ^- mtDNA, ρ^- progeny are predominantly produced due to the replicative advantage of mtDNA with large deletions. We investigated the effects of caloric restriction (CR; 0.5% glucose medium) on mitochondrial distribution and found that ρ⁺ mtDNA-mitochondria are pre-selected in zygotes and transmitted into buds prior to mitochondrial fusion. This process, termed ρ⁺ mtDN

SUBMITTER: Zhu W 

PROVIDER: S-EPMC12623940 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature