Chloroplast genome analysis reveals intraspecific variation and phylogenetic conflicts in Bletilla.

ABSTRACT: The genus Bletilla Rchb.f., an endangered taxon of Orchidaceae Juss. with significant medicinal value, faces persistent controversies in taxonomic identification and phylogenetic relationships. In this study, we analyzed a dataset of 57 complete chloroplast genomes (CPGs) from five subfamilies of Orchidaceae, including five newly sequenced Bletilla CPGs and publicly available data. These CPGs were combined with corresponding nuclear ribosomal internal transcribed spacer (nrITS) sequences obtained from public databases to resolve taxonomic uncertainties and phylogenetic relationships within Bletilla. Our results revealed significant intraspecific variation in Bletilla CPGs with overlapping intraspecific and interspecific genetic distances (GDs), alongside non-monophyletic clades of B. stria

SUBMITTER: Zhuo W 

PROVIDER: S-EPMC12624036 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature