Comparative Analysis of Species-Specific Hepatocyte Function and Drug Effects in a Liver Microphysiological System PhysioMimix LC12 and 96-Well Plates.

ABSTRACT: Drug-induced liver injury (DILI) remains a challenge in drug development, and interspecies differences in liver toxicity represent a need where comparative analyses may inform preclinical safety study design. In vitro testing for species-specific liver effects, especially in complex models such as microphysiological systems (MPS), may help predict toxicity before advancing from animal to human studies, or derisk spurious findings in preclinical species. This study assessed the utility of the perfusion-based PhysioMimix LC12 MPS as compared to 2D cultures and evaluated species-specific DILI using primary hepatocytes from human, monkey, rat, and dog. Functional, phenotypic, and transcriptional profiles were evaluated for up to 14 days. Also, cells were exposed to species-specific hepatotoxic

SUBMITTER: Negi CK 

PROVIDER: S-EPMC12624441 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature