Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal.

ABSTRACT: Safe laboratory processing requires mitigating risks from the release of pathogens into the environment through generated waste streams. This study evaluated the inactivation kinetics of bacteriophage MS2 as a surrogate for infectious viruses in liquid waste produced from total nucleic acid extractions of wastewater. The goal was to determine a waste handling protocol that ensures sufficient viral infectivity loss (i.e., inactivation) for safe disposal. Liquid waste was generated using a viral total nucleic acid extraction kit (Wizard® Enviro Total Nucleic Acid Kit, Promega, The United States of America) containing guanidinium chloride, isopropanol, ethanol, and other residual reagents. MS2 phage was artificially added into liquid waste, and inactivation was monitored over 24 h using doubl

SUBMITTER: Gan C 

PROVIDER: S-EPMC12624542 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature