Structural and dynamic roles of tandem acyl carrier proteins in aryl polyene biosynthesis in carbapenem-resistant Acinetobacter baumannii.

ABSTRACT: Aryl polyenes (APEs) are critical secondary metabolites in gram-negative pathogens, synthesized by polyketide synthases that rely on acyl carrier proteins (ACPs) as essential cofactors. Despite their importance, the structural and functional dynamics of tandem ACPs-ApeE and ApeF-have remained largely uncharacterized. In this study, we elucidate the molecular mechanisms underlying these ACPs within the APE biosynthetic gene cluster of carbapenem-resistant Acinetobacter baumannii (CRAB), a major threat to global health, revealing features that markedly differ from those in conventional fatty acid or polyketide synthesis systems. We demonstrate that ApeE functions as the primary starter ACP, engaging selectively with benzoyl-ACP synthetase (ApeH) to initiate APE biosynthesis. Through NMR spec

SUBMITTER: Lee CY 

PROVIDER: S-EPMC12624758 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature