Lipid bilayer properties govern substrate engagement and extraction by the AAA+ ATPase Msp1.

ABSTRACT: An essential aspect of protein quality control is enzymatic removal of membrane proteins from the lipid bilayer. Failures in this critical cellular process are associated with neurodegenerative diseases and cancer. Msp1 is a AAA+ (ATPases associated with diverse cellular activities) ATPase that removes mistargeted membrane proteins from the outer mitochondrial membrane. How Msp1 selectively recognizes and extracts substrates within the complex outer mitochondrial membrane ecosystem, and how the lipid bilayer impacts these processes are unknown. Here, we describe the development of a fully defined, rapid, and quantitative extraction assay that retains physiological substrate selectivity. Using this new assay, we systematically modified both the model substrate and the lipid environment to d

SUBMITTER: Fresenius HL 

PROVIDER: S-EPMC12624790 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature