A point-of-care testing device for monkeypox virus integrating recombinase polymerase amplification and lateral flow assay.

ABSTRACT:

Background

The global spread of monkeypox virus (MPXV) underscores the need for rapid, accessible diagnostic tools to control outbreaks. Current diagnostics, relying on centralized laboratory testing, require specialized personnel and equipment. We aimed to develop a point-of-care testing (POCT) kit that simplifies nucleic acid extraction, minimizes contamination, and enables immediate, accurate MPXV detection.

Methods

We introduced a novel MPXV POCT kit, featuring a separate MPXV DNA rapid release step and a closed integrated device combining recombinase polymerase amplification (RPA) and lateral flow assay (LFA). A fluorescent RPA assay identified F3L-targeting primers and probes with superior amplification efficiency. The RPA system was coupled with LFA to assess the visu

SUBMITTER: Yu J 

PROVIDER: S-EPMC12625211 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature