Cross-reactivity IgG, viral load, severity and vaccination outcome as an approach for understanding humoral immune response against SARS-CoV-2.

ABSTRACT:

Background

Serological evaluation plays a crucial role in understanding cross-reactivity, the prevalence of infection, immune response in COVID-19 disease, asymptomatic infections, and vaccine effectiveness.

Methods

Recombinant spike (rS) and Nucleocapsid (rN) proteins from SARS-CoV-2 were used to determine IgG antibodies (Abs) in serum samples obtained from Mexican adults and paediatrics before and during the pandemic by enzyme-linked immunosorbent assay.

Results

Human sera from 2003 to 2016 showed higher levels of cross seropositivity (54.5‒75%) against rS and rN. In serum samples from adult patients with COVID-19, the reactivity intensity (RI) depended on the severity of the disease, whereas in convalescent paediatric patients with COVID-19, SARS-CoV-2 viral load d

SUBMITTER: Contreras-Villa J 

PROVIDER: S-EPMC12625219 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature