Molecular features of congenital cytomegalovirus infection in neonatal mouse brain at single-cell resolution.

ABSTRACT: Cytomegalovirus is the leading viral cause of congenital infection with neurological sequelae. Effective medical treatments are limited due to an inadequate understanding of the underlying pathogenesis. Here, we applied single-cell transcriptomics to analyze neonatal mouse brains with congenital cytomegalovirus infection (cCMV). We profiled cCMV in 22 cell types and identified neural progenitor cells (NPCs) and monocyte-derived macrophages (MDMs) as the most commonly infected cells. Infected NPCs exhibited dysregulated neurodevelopment-associated signaling pathways, correlating with viral transcript levels that indicate viral replication levels. Genes associated with phagocytosis and antigen presentation were downregulated exclusively in infected MDMs but remained largely unaffected in mic

SUBMITTER: Mei MJ 

PROVIDER: S-EPMC12625350 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature