CBX7 regulates chemotherapy-induced senescence-like growth arrest in multiple myeloma via the ERK/STAT3/PIM1 axis.

ABSTRACT:

Background

Chemotherapy incorporating the proteasome inhibitor bortezomib (BTZ) has improved outcomes for patients with multiple myeloma (MM); however, resistance to chemotherapy and disease relapse remain significant challenges, closely associated with cellular senescence. This study investigated the key drivers of myeloma cell senescence and its role in MM progression.

Methods

Flow cytometry assessed senescence-associated β-galactosidase (SA-β-gal) activity in myeloma cells from bone marrow samples of MM patients. BTZ was used to establish cell senescence model. RNA-seq identified key genes regulating myeloma cell senescence, which were verified by qPCR. After CBX7 knockdown and overexpression, SA-β-gal staining, CCK-8 assay, cell cycle assay, and colony formation assay we

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12625351 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature