Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) exert therapeutic effects primarily through their secretome, rich in bioactive factors with immunomodulatory and regenerative properties. However, clinical application of MSC-derived secretome is hindered by donor variability, limited expansion, and replicative senescence. To address these issues, we developed immortalized human hair follicle-derived mesenchymal-like stromal cells (iHF-MSCs) as a consistent and scalable source of therapeutic secretome.

Methods

HF-MSCs were isolated using a minimally invasive procedure and immortalized with lentiviral SV40 large T antigen. From 576 single-cell clones, C18 and C26 were selected based on proliferation capacity, absence of senescence, and retention of a mesenchymal-like phenotype. Char

SUBMITTER: Rossello-Gelabert M 

PROVIDER: S-EPMC12625642 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature