Exogenous glutamate potentiates gentamicin to kill multidrug- and carbapenem-resistant <i>Pseudomonas aeruginosa</i> by activating the biosynthesis of unsaturated fatty acids.

ABSTRACT: Multidrug- and carbapenem-resistant Pseudomonas aeruginosa (MDR-PA and CR-PA) are difficult to control due to the predicament caused by their limited membrane permeability. The metabolic reprogramming approach is an effective strategy to promote membrane permeability. In this study, a gas chromatography-mass spectrometer-based metabolomics identified decreased abundance of glutamate as the most characteristic feature in gentamicin-resistant P. aeruginosa (PA-R_GEN). Exogenous glutamate enhanced gentamicin killing to lab-evolved PA-R_GEN as well as clinical MDR-PA and CR-PA isolates. By applying a multi-faceted approach, including glutamate-reprogramming metabolomics, isotope-tracing analysis, glutamate-reprogramming lipidomics, membrane permeability measur

SUBMITTER: Lai H-F 

PROVIDER: S-EPMC12625761 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature