HIV-1 Vif and Vpr cooperatively modulate the cell cycle to maximize per-cell virion production.

ABSTRACT: The HIV type 1 (HIV-1) accessory proteins Virion Infectivity Factor (Vif) and Viral Protein R (Vpr) are essential for efficient virus replication in vivo. Vif mediates degradation of host restriction factor APOBEC3G, while Vpr modulates the host cell proteome in ways that promote virion infectivity and viral gene expression. In cycling cells including CD4+ T cells, Vif and Vpr also severely impact cell cycle progression for reasons that remain unknown. Here, we combined live-cell imaging with virological assays to define the relative impacts of Vif and Vpr on the cell cycle and single-cell virion production. We demonstrate that Vif and Vpr's effects on the cell cycle are markedly distinct, with Vif arresting cells in mitosis, while Vpr causes a G2 phase delay followed by endoreplication an

SUBMITTER: Bandini M 

PROVIDER: S-EPMC12626010 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature